Polymerase chain reaction assay identifies Culex nigripalpus: part of an assay for molecular identification of the common Culex (Culex) mosquitoes of the eastern United States.

نویسندگان

  • Stephen Aspen
  • Mary B Crabtree
  • Harry M Savage
چکیده

Nucleotide sequence information on internal transcribed spacer (ITS) 1 and ITS 2 regions of the nuclear ribosomal DNA multigene family was used to develop a polymerase chain reaction assay that identifies Culex nigripalpus Theobald. The assay uses species-specific forward and reverse primers for Cx. nigripalpus and can be used along with previously described primers to distinguish among 4 common taxa of Culex (Culex) of the eastern USA with a single thermal cycler program. The assay distinguishes among the 4 taxa Cx. nigripalpus, Cx. restuans Theobald, Cx. salinarius Coquillett, and members of the Cx. pipiens Linnaeus complex. This assay may be used to verify the morphological identification of individual specimens of Culex or to confirm the species composition of mosquito pools.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Development of a species-diagnostic polymerase chain reaction assay for the identification of Culex vectors of St. Louis encephalitis virus based on interspecies sequence variation in ribosomal DNA spacers.

Culex pipiens complex mosquitoes (Cx. p. pipiens and Cx. p. quinquefasciatus) are among the principal vectors of St. Louis encephalitis (SLE) virus in the eastern United States; Cx. restuans and Cx. salinarius play secondary roles in the transmission and maintenance of the virus cycle. Accurate identification of these three species in field collections is required for epidemiologic studies of S...

متن کامل

MORPHOLOGY, SYSTEMATICS, EVOLUTION Development of Multiplexed Species Specific Polymerase Chain Reaction Assays for Identification of the Culex (Melanoconion) Species (Diptera: Culicidae) of the Southeastern United States Based on rDNA

Adult female mosquitoes within the subgenus Culex (Melanoconion) Theobald (Diptera: Culicidae) are difÞcult to identify to species using external morphological features. We present two multiplexed polymerase chain reaction assays that quickly and accurately identify specimens from the southeastern United States based on sequence differences in the internal transcribed spacers of the ribosomal D...

متن کامل

Host feeding patterns of potential vectors of eastern equine encephalitis virus at an epizootic focus in Tennessee.

In 2006, 2,817 blood-fed mosquitoes were collected from the site of a 2005 eastern equine encephalitis outbreak in Chester County, TN. Using a polymerase chain reaction-based assay, 264 vertebrate hosts were identified from seven mosquito species. Culex erraticus and Cx. nigripalpus fed on a diversity of mammalian, avian, and reptilian hosts, whereas Anopheles quadrimaculatus and An. punctipenn...

متن کامل

A study of the distribution of the Culex pipiens complex (Insecta: Diptera: Culicidae) mosquitoes in the European part of Russia by molecular methods of identification

Three members of the Culex pipiens complex mosquitoes, Culex pipiens L. (including the pipiens and molestus forms) and Culex torrentium Martini, occur in the European part of Russia. The geographical and biotopic distribution of these mosquitoes was studied using molecular-genetic methods for their identification. They included Wolbachia infection, sizes of ITS2 of rDNA, polymerase chain reacti...

متن کامل

Identification of Aedes aegypti and its respective life stages by real-time polymerase chain reaction.

An Aedes aegypti-specific, fluorogenic probe hydrolysis (Taq-Man), polymerase chain reaction assay was developed for real-time screening using a field-deployable thermocycler. Laboratory-based testing of A. aegypti, A. aegypti (Trinidad strain), Culex pipiens, Culex quinquefasciatus, Anopheles stephensi, and Ochlerotatus taeniorhynchus individual adult mosquitoes and mixed pools (n = 10) demons...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of the American Mosquito Control Association

دوره 19 2  شماره 

صفحات  -

تاریخ انتشار 2003